Research Assistant II

The University of Texas MD Anderson Cancer Center is one of the world's leading cancer care institutions. They are seeking a Research Assistant II to provide technical and organizational support for ongoing research projects, including assisting with manuscript and grant preparation. Responsibilities Requires basic knowledge and techniques to conduct various experiments in molecular biology and protein chemistry An essential requirement for the job is tissue/cell culture, molecular cloning, and real time PCR techniques, with the ability to culture/maintain different cancer cell lines, to subclone DNA expression plasmid and to carry out mutagenesis experiments Also critical is knowledge of protein overexpression, basic protein purification and analysis techniques (e.g. Western blotting, etc.) Requires handling experimental mice, including injecting tumor cells to nude mice, measure tumor growth, harvest tumor samples, isolate DNA and RNA from tumors, and carry out immunohistochemistry using paraffin tissue sections Must be willing and able to learn and conduct additional methods and techniques when necessary If necessary, be able to keep flexible working hours and to learn proper procedures to operate various types of equipment associated with these experiments Required to record and maintain a central repository of all molecular biology reagents and biospecimens Accurately label all samples Keep ample stock of supplies on hand Skills Bachelor's degree with major course works in one of the basic sciences One year experience in scientific or experimental research work Basic knowledge and techniques to conduct various experiments in molecular biology and protein chemistry Ability to culture/maintain different cancer cell lines Ability to subclone DNA expression plasmid Ability to carry out mutagenesis experiments Knowledge of protein overexpression Basic protein purification and analysis techniques (e.g. Western blotting, etc.) Handling experimental mice, including injecting tumor cells to nude mice Measure tumor growth Harvest tumor samples Isolate DNA and RNA from tumors Carry out immunohistochemistry using paraffin tissue sections Willingness and ability to learn and conduct additional methods and techniques when necessary Ability to keep flexible working hours Learn proper procedures to operate various types of equipment associated with these experiments Record and maintain a central repository of all molecular biology reagents and biospecimens Accurately label all samples Keep ample stock of supplies on hand Master's degree Animal work Cell culture Experiment design Benefits Medical Dental Paid time off Retirement Tuition benefits Educational opportunities Individual and team recognition Company Overview Through generous funding from Susan G. It was founded in undefined, and is headquartered in Houston, Texas, US, with a workforce of 11-50 employees. Its website is